厙ぴ勛圖

• Gram-negative bacteria (Escherichia coli), Gram-positive bacteria (Bacillus subtilis), yeast (Pichia pastoris) and mammalian (HEK293) expression
• Regulatory approval and access to fermentation capabilty for expression of proteins and biological materials in all common microbial species up to 1000-L scale
• Suite of expression vectors, chaperones, and strains
• Parallel expression of protein fusions for purification, detection and solubility
• Fusion tag removal with HRV3C, TEV and SUMO proteases

• Antibody engineering for production of recombinant antibodies, nanobodies, light chains, chimeric mouse/human antibodies, Fv and Fab antibody fragments, recombinant immune complexes and polymeric IgGs
• Hybridoma maintenance and cloning
• Antibody purification and isotyping
• Epitope mapping using random epitope and peptide libraries
• Isolation and recombinant expression of antibody genes from hybridomas

• In-house modular DNA assembly platform
• Combinatorial gene assembly from libraries of promoters, terminators, etc.
• Multigene assembly
• Vector design and construction
• Metabolic pathway construction
• Site directed mutagenesis
• PCR and DNA sequencing
• Quantitative PCR
• DNA and RNA extraction
• Fluorometric high sensitivity DNA and RNA quantification
• Agarose gel electrophoresis

• Cell fractionation
• Fast protein liquid chromatography (FLPC) including affinity chromatography, ion exchange chromatography and size exclusion chromatography (SEC)
• Hydrophobic interaction chromatography (HIC)
• Protein refolding
• Desalting
• Freeze drying

• Protein electrophoresis (SDS-PAGE)
• Isoelectric focusing (IEF)
• Western blotting
• Protein concentration measurement by bicinchoninic acid, Lowry, Bradford and UV
• Structural integrity by circular dichroism (CD)
• Thermal stability by differential scanning calorimetry (NanoDSC)
• Biomolecular interactions by isothermal calorimetry (ITC)
• Mass spectroscopy (total mass and peptide mapping)
• Kinetic analysis of biomolecular interactions using surface plasmon resonance and bio-layer interferometry
• X-ray crystallography
• Particle size analysis by light scattering and analytical ultracentrifugation

• Spectroscopy (far UV, near UV, infrared, fluorescence)
• Gas chromatography(GC-FID, GC-MS)
• High performance liquid chromatography (HPLC/UPLC-variable wavelength/CAD)
• Nuclear magnetic resonance (1D and 2D)
• Mass spectroscopy

• Enzyme and coupled enzyme assays (colourimetric, UV, fluorescence, luminescence)
• High performance liquid chromatography
• ELISA (direct, indirect, sandwich, competition & inhibition) for protein quantitation and study of protein-protein interactions
• Zeta potential
• Endotoxin measurement
• Chemical quantitation assays
• High performance liquid chromatography (HPLC/UPLC-variable wavelength/CAD)

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• Anti-inflammatory assays (cytochrome oxidase and lipoxygenase, prostaglandin ELISA suite)
• Confocal Microscopy
• Reporter gene assays

In addition to R&D services, we also offer

• Scientific consultancy
• Literature and patent reviews
• Project management
• Industry placement and technology transfer
• Coordination of access to complementary research services